Role of Bacillus subtilis SpoIIIE in DNA Transport Across the Mother Cell-Prespore Division Septum
- 3 November 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 290 (5493) , 995-997
- https://doi.org/10.1126/science.290.5493.995
Abstract
The SpoIIIE protein of Bacillus subtilis is required for chromosome segregation during spore formation. The COOH-terminal cytoplasmic part of SpoIIIE was shown to be a DNA-dependent adenosine triphosphatase (ATPase) capable of tracking along DNA in the presence of ATP, and the NH 2 -terminal part of the protein was found to mediate its localization to the division septum. Thus, during sporulation, SpoIIIE appears to act as a DNA pump that actively moves one of the replicated pair of chromosomes into the prespore. The presence of SpoIIIE homologs in a broad range of bacteria suggests that this mechanism for active transport of DNA may be widespread.Keywords
This publication has 14 references indexed in Scilit:
- Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co‐localization with the Spo0J partitioning proteinMolecular Microbiology, 1997
- Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilisCell, 1997
- Postseptational chromosome partitioning in bacteria.Proceedings of the National Academy of Sciences, 1995
- A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.Genes & Development, 1995
- Bacillus subtilis spoIIIE Protein Required for DNA Segregation During Asymmetric Cell DivisionScience, 1994
- Template Supercoiling by a Chimera of Yeast GAL4 Protein and Phage T7 RNA PolymeraseScience, 1990
- Template supercoiling during ATP-dependent DNA helix tracking: studies with simian virus 40 large tumor antigen.Proceedings of the National Academy of Sciences, 1989
- Transcription-driven supercoiling of DNA: Direct biochemical evidence from in vitro studiesCell, 1989
- Supercoiling of the DNA template during transcription.Proceedings of the National Academy of Sciences, 1987
- Interaction between DNA and an Escherichia coli protein ωJournal of Molecular Biology, 1971