Abstract
The amino acid requirements of developing honeybee larvae were determined by the indirect method using glucose-U-14C and sucrose-U-14C. The amino acids proline, hydroxyproline, alanine, glutamine, glutamic acid, α-amino-n-butyric acid, γ-amino-n-butyric acid, glycine, serine, and β-alanine were classified as nonessential. Cystine, aspartic acid, asparagine, isoleucine, leucine, phenylalanine, methionine, tryptophan, valine, threonine, tyrosine, lysine, histidine, and arginine were considered to be essential. The metabolism of sugar and aspartic acid are discussed in relation to caste development.