Improving de Novo Sequencing of Peptides Using a Charged Tag and C-Terminal Digestion
- 20 January 2007
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (4) , 1583-1590
- https://doi.org/10.1021/ac061670b
Abstract
An improved method for peptide de novo sequencing by MALDI mass spectrometry is presented. The method couples a charge derivatization reaction with C-terminal digestion to modify tryptic peptides. The charge derivatization attaches a fixed charge group onto the N-termini of peptides, and the enzymatic digestion after the derivatization step removes C-terminal basic amino acid residues such as arginine and lysine. The fragmentation of the modified peptide(s) under low-energy CID conditions (MALDI Q-TOF mass spectrometer) yields a simplified yet complete ion series of the peptide sequence. The validity of the method is demonstrated by the results from several model protein digests, where peptide sequences were correctly deduced either manually or through an automated sequencing program.Keywords
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