Screening Sanitizing Agents and Methods of Application for Hatching Eggs II. Effectiveness Against Microorganisms on the Egg Shell

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Abstract
Twenty three sanitizers were tested for their effectiveness against a “cocktail” of inoculated microorganisms on the egg shell surface. Following inoculation, the eggs were sanitized (using either three concentrations of exposure times per sanitizer), and a sample of a rinse solution from individual eggs was plated on commercially produced biplate media slides (nutrient agar on one side and MacConkey agar on the other). The slides were incubated (37°C) for 48 hours and total colonies were counted. The procedure provided a quick and economical method of assessing the overall effectiveness of a sanitizer against a variety of microorganisms on the egg shell surface. However, the method did not accurately differentiate between either concentration or exposure time of individual sanitization treatments.