REGULATION OF HUMAN GRANULOCYTE FUNCTION BY PRODUCTS DERIVED FROM MURINE TUMORS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 13  (2) , 151-156
Abstract
Murine EL-4 thymoma was found to produce a factor that activated antibody-dependent cell-mediated cytotoxicity by human eosinophils and neutrophils. The eosinophil-activating factor had some properties similar to human eosinophil colony-stimulating factor [CSF] in being heat stable, inactivated by 2-mercaptoethanol, and cochromatographing by gel filtration with a factor that promoted the growth of pure eosinophil colonies from human bone marrow. The neutrophil-activating factor from EL-4 was also heat stable but was not inactivated by 2-mercaptoethanol and was found in 2 peaks on chromatography, neither having human neutrophil-CSF activity but 1 cochromatographing with mouse granulocyte-macrophage CSF. EL-4 secretes factors that activate 2 types of human granulocytes. Several other murine thymomas and macrophage cell lines produced a neutrophil-activating factor, the production of which could not be correlated with that of several other known lymphokines. Murine tumors produce factors that activate human granulocytes.