Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protein kinase C activation
- 30 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 243 (2) , 275-279
- https://doi.org/10.1016/0014-5793(89)80144-9
Abstract
Aspirin-pretreated, 32P-prelabeled, washed human platelets resuspended in a buffer containing apyrase and 2% plasma were exposed to epinephrine and the Ca2+ ionophore A23187. Epinephrine potentiated platelet aggregation (not secretion), the production of [32P]phosphatidic acid and myosin light chain phosphorylation induced by A23187. No phosphorylation of the 40 kDa protein, the substrate of protein kinase C, was observed. We conclude that G1-protein activation evoked by epinephrine and Ca2+ mobilization caused by A23187 represents a novel synergism for platelet aggregation and that protein kinase C activation, under these conditions is not needed for platelet aggregation.Keywords
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