Sample preparation of human tumors prior to two‐dimensional electrophoresis of proteins
- 1 January 1995
- journal article
- Published by Wiley in Electrophoresis
- Vol. 16 (1) , 1087-1089
- https://doi.org/10.1002/elps.11501601184
Abstract
Sample preparation procedures are critical for obtaining high quality two‐dimensional electrophoresis (2‐DE) separations from clinical tumor materials. In this communication, we describe procedures which we have found satisfactory for preparing various tumor samples for 2‐DE. By these procedures, tumor cells are enriched from serum proteins and contaminating stromal cells. Tumors can be prepared with good reproducibility to obtain high resolution using these techniques.Keywords
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