Integration Site forStreptomycesPhage φBT1 and Development of Site-Specific Integrating Vectors
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- 1 September 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (17) , 5320-5323
- https://doi.org/10.1128/jb.185.17.5320-5323.2003
Abstract
Despite extensive similarities between the genomes of the Streptomyces temperate phages φC31 and φBT1, the attP-int loci are poorly conserved. Here we demonstrate that φBT1 integrates into a different attachment site than φC31. φBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on φBT1 integrase were shown to have a broad host range and are fully compatible with those based on the φC31 attP-int locus.Keywords
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