STUDIES in phloridzinized animals were responsible for some of the earliest indications that the adrenal steroids play an important role in the regulation of gluconeogenesis (Wells, 1940, and Wells and Kendall, 1940). Unfortunately, at the time of these early studies, no good preparations of adrenocorticotrophic hormone (ACTH) were available. Experiments assessing the effectiveness of these steroids on ketone excretion were also performed but usually in animals other than those used in the glucose and nitrogen studies and under somewhat different experimental conditions (Wells and Kendall, 1941). The present investigation was undertaken in an attempt to evaluate the role of the adrenal in gluconeogenesis by administration of individual adrenal steroids to adrenalectomized phloridzinized rats and observation of their effects in graded doses upon glucose, nitrogen and ketone excretion. This was also done in intact animals given ACTH.