Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: The 5S rRNA gene is separated from the 16S and 23S rRNA genes
- 1 December 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 205 (3) , 428-433
- https://doi.org/10.1007/bf00338078
Abstract
In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors λEMBL3 and λEMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was generated and the organization of the rRNA genes was determined. The results show that the genes for the 16S and 23S rRNAs are closely spaced and occur only once in the genome, whereas the 5S rRNA gene is separated from the other two genes by more than 4 kb.Keywords
This publication has 33 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsPublished by Elsevier ,2006
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- REGULATION OF THE SYNTHESIS OF RIBOSOMES AND RIBOSOMAL COMPONENTSAnnual Review of Biochemistry, 1984
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilisGene, 1982
- A very large repeating unit of mouse DNA containing the 18S, 28S and 5.8S rRNA genesCell, 1977
- The number of rRNA genes in Escherichia coliFEBS Letters, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969