Expression of levansucrase- -galactosidase hybrids inhibits secretion and is lethal in Bacillus subtilis
- 1 June 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 136 (6) , 1137-1143
- https://doi.org/10.1099/00221287-136-6-1137
Abstract
Summary: The lacZ gene of Escherichia coli was fused to several positions downstream from the 5” end of the Bacillus subtilis sacB gene, which encodes levansucrase (LS), a sucrose-inducible extracellular enzyme. Effects of hybrid protein expression in B. subtilis were studied. Several fusions were tested, and two significantly interfered with growth of cells and with LS secretion when induced with sucrose. Chromosomal amplification of the fusions, leading to strong expression of the hybrid proteins, completely blocked LS secretion and was lethal for B. subtilis when expression was induced.This publication has 19 references indexed in Scilit:
- The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sitesMolecular Genetics and Genomics, 1985
- Secretory S complex of Bacillus subtilis forms a large, organized structure when released from ribosomes.Proceedings of the National Academy of Sciences, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Hyperproduction of Exocellular Levansucrase by Bacillus subtilis: Examination of the Phenotype of a sacUh StrainMicrobiology, 1984
- Invertase beta-galactosidase hybrid proteins fail to be transported from the endoplasmic reticulum in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1984
- Sequence information within the lamB gene is required for proper routing of the bacteriophage λ receptor protein to the outer membrane of Escherichia coli K-12Journal of Molecular Biology, 1982
- E. coli mutant pleiotropically defective in the export of secreted proteinsCell, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILISJournal of Bacteriology, 1961