Indirect Ultraviolet Visualization and Quantitation of Methenamine with Reversed Phase Ion Pairing Liquid Chromatography
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 16 (9) , 711-721
- https://doi.org/10.1080/00032718308065207
Abstract
Indirect UV visualization of a polycyclic polyamine, methenamine, a urinary antibacterial agent, is reported for the 1st time. The ability to detect a non-UV active compound quantitatively using a UV monitor is demonstrated; the optimized experimental conditions are discussed. The calibration curve obtained was linear over 2 orders of magnitude extending to a detection limit of 250 pmol.This publication has 4 references indexed in Scilit:
- Quantitation of Alkyl Sulfonates Using UV Detector Sensitive “Ion-Pair” Reagents in Reversed-Phase Liquid ChromatographyJournal of Liquid Chromatography, 1982
- Reversed-phase ion-pair chromatography with UV-absorbing ions in the mobile phaseJournal of Chromatography A, 1981
- Separation of Ionic Compounds by Reversed-Phase Liquid Chromatography An Update of Ion-Pairing TechniquesJournal of Chromatographic Science, 1980
- Determination of free and conjugated bile acidsas ultraviolet-absorbing lon pairs by reverse-phase high-performance liquid chromatographyAnalytical Biochemistry, 1979