Perturbation of D 1 Dopamine and AT 1 Receptor Interaction in Spontaneously Hypertensive Rats
- 1 October 2003
- journal article
- research article
- Published by Wolters Kluwer Health in Hypertension
- Vol. 42 (4) , 787-792
- https://doi.org/10.1161/01.hyp.0000085334.34963.4e
Abstract
The dopaminergic and renin-angiotensin systems interact to regulate blood pressure. Because this interaction may be perturbed in genetic hypertension, we studied D1 dopamine and AT1 angiotensin receptors in immortalized renal proximal tubule (RPT) and A10 aortic vascular smooth muscle cells. In normotensive Wistar-Kyoto (WKY) rats, the D1-like agonist fenoldopam increased D1 receptors but decreased AT1 receptors. These effects were blocked by the D1-like antagonist SCH 23390 (10−7 mol/L per 24 hours). In spontaneously hypertensive rat (SHR) RPT cells, fenoldopam also decreased AT1 receptors but no longer stimulated D1 receptor expression. Basal levels of AT1/D1 receptor coimmunoprecipitation were greater in WKY RPT cells (29±2 density units, DU) than in SHR RPT cells (21±2 DU, n=7 per group, P<0.05). The coimmunoprecipitation of D1 and AT1 receptors was increased by fenoldopam (10−7 mol/L per 24 hours) in WKY RPT cells but decreased in SHR RPT cells. The effects of fenoldopam in RPT cells from WKY rats we...Keywords
This publication has 41 references indexed in Scilit:
- Angiotensin II Regulation of AT 1 and D 3 Dopamine Receptors in Renal Proximal Tubule Cells of SHRHypertension, 2003
- Gα 12 - and Gα 13 -Protein Subunit Linkage of D 5 Dopamine Receptors in the NephronHypertension, 2003
- Biphasic Regulation of Na + -HCO 3 − Cotransporter by Angiotensin II Type 1A ReceptorHypertension, 2002
- Desensitization of human renal D1 dopamine receptors by G protein-coupled receptor kinase 4Kidney International, 2002
- Differential Dependence of the D1 and D5Dopamine Receptors on the G Protein γ7 Subunit for Activation of AdenylylcyclasePublished by Elsevier ,2001
- Impaired Renal Vascular Response to a D1-Like Receptor Agonist But Not to an ACE Inhibitor in Conscious Spontaneously Hypertensive RatsJournal of Cardiovascular Pharmacology, 1999
- Disruption of the dopamine D3 receptor gene produces renin-dependent hypertension.Journal of Clinical Investigation, 1998
- Dopamine decreases expression of type-1 angiotensin II receptors in renal proximal tubule.Journal of Clinical Investigation, 1996
- Two distinct pathways in the down-regulation of type-1 angiotensin II receptor gene in rat glomerular mesangial cellsBiochemical and Biophysical Research Communications, 1992
- Characterization of two putative smooth muscle cell lines from rat thoracic aortaExperimental Cell Research, 1976