Production of leukemic blast growth factor by a human bladder carcinoma cell line
Open Access
- 1 September 1985
- journal article
- Published by American Society of Hematology in Blood
- Vol. 66 (3) , 748-751
- https://doi.org/10.1182/blood.v66.3.748.748
Abstract
Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.Keywords
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