Antibody-Mediated Target Cell Lysis by Nonimmune Cells: The Use of Anti-Immunoglobulin to Distinguish Effector Cell Populations
Open Access
- 1 February 1974
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 112 (2) , 792-803
- https://doi.org/10.4049/jimmunol.112.2.792
Abstract
The role of a goat IgG anti-rabbit immunoglobulin on antibody-mediated target cell lysis by nonimmune cells was investigated. Using a homologous system of rabbit effector cells and rabbit anti-fowl erythrocyte serum, two populations of cells effective in the lysis of antibody-coated target cells could be differentiated by anti-immunoglobulin. In the presence of the anti-immunoglobulin, the cytotoxicity caused by phagocytic cells was suppressed. The IgG or (Fab′)2 fraction of the anti-immunoglobulin enhanced target cell destruction initiated by purified lymph node and spleen lymphocytes and to a lesser extent that initiated by purified thymus cells. Monovalent (Fab) anti-immunoglobulin decreased the cytotoxic activity of all effector cells. The mechanism of enhancement appeared to be due to cross-linking between effector lymphocytes and antibody-coated target cells by the divalent anti-immunoglobulin.Keywords
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