FREEZE-FRACTURE STUDIES OF THE PLASMALEMMA OF CANDIDA-ALBICANS AFTER TREATMENT WITH ECONAZOLE-NITRATE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 17  (4) , 389-398
Abstract
In EM studies the interior of the plasmalemma of C. albicans was revealed by the freeze-fracture technique. Superficial structures of the extracellular (E) and protoplasmic (P) fracture faces differed negligibly from structures on the corresponding fracture faces of Saccharomyces cerevisiae. Following treatment with 2.2 .times. 10-5 M econazole nitrate, a layer present on the P face in the form of a tight matrix of globular proteins dissolved into isolated groups of particles whose globular elements sometimes formed hexagonal patterns. As the damage progressed, fissure-shaped membrane invaginations on the P face disappeared. Parts of the outer lipid layer of the plasmalemma were torn off the cell wall and adhered in fragments to the P face. Ultrastructural changes in the plasmalemma induced by econazole nitrate temporally correlate with an increase in permeability of the cell envelope found in physiological studies.

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