Simple transphosphatidylation of phospholipids catalysed by a lipid-coated phospholipase D in organic solvents

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Abstract
A lipid-coated phospholipase D (PLD) was prepared by mixing aqueous solutions of PLD and lipids. The lipid-coated PLD showed a high catalytic activity for transphosphatidylation of egg yolk phosphatidyl-choline (egg-PC) with alcohols in two-phase benzene–acetate buffer solution. Since both substrates and enzymes are soluble in the organic phase, the reaction proceeded in the benzene phase and the aqueous phase is required to remove the produced choline moiety from the organic phase. When a native PLD was employed instead of the lipid-coated PLD, the reaction was very slow (∼ 1/300 that of the lipid-coated PLD) because the reaction occurs at the interface of the lipophilic substrates and water-soluble enzymes. The transphosphatidylation catalysed by a lipid-coated PLD could be applied in a manner widely independent of the nature of the head groups of the coating lipids and the polarity of the organic solvents, and could be applied also on a large-scale (yield 1–2 g) synthesis to introduce various alcohols, sugars, and nucleic acids at the head groups of phospholipids. We have determined substrate selectivity and Michaelis–Menten kinetics for a lipid-coated PLD and compared the results with those for the native PLD.

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