Appearance of a functional insulin receptor during rabbit embryogenesis
- 1 June 1985
- journal article
- research article
- Published by Springer Nature in Diabetologia
- Vol. 28 (6) , 369-372
- https://doi.org/10.1007/bf00283146
Abstract
The domain structure of the insulin receptor was investigated in liver and brown adipose tissue of developing rabbits. The structure of the binding domain (α-subunit) was analysed after covalent labelling with a 125I photo-reactive insulin analogue. The structure of the tyrosine kinase domain (β-subunit) and the transmission of the hormonal signal from the α-to the β-subunit were analysed by stimulating with insulin the autophosphorylation of the β-subunit. Finally, the immunoreactivity of the receptor in developing tissues was assessed with anti-receptor antibodies. The results show that a functional insulin receptor can be detected at the early stages of fetal development in both tissues and is conserved throughout ontogenesis to adulthood.Keywords
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