Endothelial nitric oxide synthase localized to hippocampal pyramidal cells: implications for synaptic plasticity.
Open Access
- 10 May 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (10) , 4214-4218
- https://doi.org/10.1073/pnas.91.10.4214
Abstract
Using antibodies that react selectively with peptide sequences unique to endothelial nitric oxide synthase (eNOS), we demonstrate localizations to neuronal populations in the brain. In some brain regions, such as the cerebellum and olfactory bulb, eNOS and neuronal NOS (nNOS) occur in the same cell populations, though in differing proportions. In the hippocampus, localizations of the two enzymes are strikingly different, with eNOS more concentrated in hippocampal pyramidal cells than in any other brain area, whereas nNOS is restricted to occasional interneurons. In many brain regions NADPH diaphorase staining reflects NOS catalytic activity. Hippocampal pyramidal cells do not stain for diaphorase with conventional paraformaldehyde fixation but stain robustly with glutaraldehyde fixatives, presumably reflecting eNOS catalytic activity. eNOS in hippocampal pyramidal cells may generate the NO that has been postulated as a retrograde messenger of long-term potentiation.Keywords
This publication has 56 references indexed in Scilit:
- Cerebral endothelial nitric oxide synthase expression after focal cerebral ischemia in rats.Stroke, 1993
- The suppression of long-term potentiation in rat hippocampus by inhibitors of nitric oxide synthase is temperature and age dependentNeuron, 1993
- Neurons synthesizing nitric oxide innervate the mammalian carotid bodyJournal of Comparative Neurology, 1993
- Activation of Nitric Oxide Synthase from Cultured Aortic Endothelial Cells by PhospholipidsBiochemical and Biophysical Research Communications, 1993
- Nitric Oxide Synthases in Neuronal Cells, Macrophages and Endothelium Are NADPH Diaphorases, but Represent Only a Fraction of Total Cellular NADPH Diaphorase ActivityBiochemical and Biophysical Research Communications, 1993
- Expression of Nitric-Oxide Synthase (NOS) in Injured CNS Neurons as Shown by NADPH Diaphorase HistochemistryExperimental Neurology, 1993
- Nitric Oxide: First in a New Class of NeurotransmittersScience, 1992
- Nitric oxide synthase protein and mRNA are discretely localized in neuronal populations of the mammalian CNS together with NADPH diaphoraseNeuron, 1991
- Glutamate, nitric oxide and cell-cell signalling in the nervous systemTrends in Neurosciences, 1991
- Localization of nitric oxide synthase indicating a neural role for nitric oxideNature, 1990