Dechlorosoma suillum Achenbach et al. 2001 is a later subjective synonym of Azospira oryzae Reinhold-Hurek and Hurek 2000
Open Access
- 1 July 2003
- journal article
- research article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 53 (4) , 1139-1142
- https://doi.org/10.1099/ijs.0.02606-0
Abstract
In order to clarify the taxonomic position of Dechlorosoma suillum, which shares 99·9 % 16S rDNA sequence identity (1433 of 1435 bp) with Azospira oryzae, we compared the two species in a polyphasic taxonomic approach. Results of 122 physiological and biochemical tests for D. suillum DSM 13638T and Azospira oryzae 6a3T were identical, except for the lack of growth of Azospira oryzae 6a3T with perchlorate as the terminal electron acceptor. Presence of a nifH gene and nitrogenase activity, a key feature of Azospira, were also detected in D. suillum by Southern hybridization and by the acetylene reduction assay, respectively. Whole-cell SDS-PAGE profiles of SDS-soluble proteins of strains DSM 13638T and 6a3T were almost identical. DNA–DNA hybridization studies showed more than 90 % binding between D. suillum and two strains of Azospira oryzae. These data provide evidence that the two bacteria belong to the same species and that D. suillum is a later subjective synonym of Azospira oryzae.Keywords
This publication has 17 references indexed in Scilit:
- Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of DechloromonasNature, 2001
- Dechloromonas agitata gen. nov., sp. nov. and Dechlorosoma suillum gen. nov., sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and their phylogenetic position.International Journal of Systematic and Evolutionary Microbiology, 2001
- Preferential occurrence of diazotrophic endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with modern racesEnvironmental Microbiology, 2000
- Reduction of (per)chlorate by a novel organism isolated from paper mill wasteEnvironmental Microbiology, 1999
- Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the beta subclassJournal of Bacteriology, 1997
- Global changes in protein composition of N2-fixing-Azoarcus sp. strain BH72 upon diazosome formationJournal of Bacteriology, 1996
- 16S rRNA-targeted polymerase chain reaction and oligonucleotide hybridization to screen for Azoarcus spp., grass-associated diazotrophsApplied and Environmental Microbiology, 1993
- Numerical Methods in the Classification of Bacteria by Protein ElectrophoresisPublished by Elsevier ,1985
- The Quantitative Measurement of DNA Hybridization from Renaturation RatesEuropean Journal of Biochemistry, 1970
- Evolution of Protein MoleculesPublished by Elsevier ,1969