Study on the separation and characterization of cadmium incorporated intoZea Mays L.

Abstract
Methods of separation and characterization of Cd in Z. mays were studied by a radioisotopic technique based on injecting 109Cd into the plant. Two classic methods for fractionating plant material were compared. Cd was found in all different cell components. A significant activity of Cd in the intracellular liquid was bound to proteins as indicated by chromatogrphy on a molecular sieve. Cd fixed to cell walls exchanges entirely after selective solvent extraction with H2O, KNO3, HAc [acetic acid], HCl. Cd-binding sites in stems was indicated.