Reconstruction of Dissociated Chick Brain Cells in Rotation-Mediated Culture

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Abstract
Using rotation-mediated cultivationl cells dissociated from brain of 6- to 9-day chick embryos were cultured in vitro. Both cerebral and midbrain cells dissociated from successively older embryos made progressively smaller aggregates. Observation of histogenesis in the aggregates suggests that the decrease in the size of aggregates is related to the cellular differentiation. In Eagle''s basal medium containing bovine serum in various concentrations, largest aggregates were formed with 10% or 20% serum. Aggregates cultured in Eagle''s basal medium alone were similar in size to those cultured with 5% or 40% serum. These results indicated that serum seems to have no direct effect on the cell-to-cell contact, although it may be necessary for general cell metabolism. Aggregates of the cerebrum, the midbrain, and the neural-retina cells from 7 1/2-day chick embryos were cultured. Aggregates of cerebral cells and midbrain cells showed an increase in size, probably due to cell growth, or the formation of fibers. Aggregates of neural-retina cells showed changes in size produced by the fusion or fragmentation of the aggregates. Histogenesis of commingled aggregates of cerebral and neural-retina cells shows the neural-retina tissue is arranged concentrically around the cerebral tissue. This suggests that a sorting out took place between the cerebral cells and the neural-retina cells.