Indirect UV Detection of Organic Analyte Anions Using a Low-Capacity Anion Exchange Column

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Abstract
Organic analyte anion retention on a low-capacity anion exchange column using indirect UV detection was studied. A combination of anion exchange/reversed-phase interactions were found to influence the retention of organic analyte anions provided the analytes have both an anionic charge site and a hydrophobic center. Organic analyte anion retention was found to be influenced by the following: concentration of organic modifier, concentration of UV absorbing analyte, pH, and mobile phase ionic strength. Correlation coefficients of better than 0.999 were found for several of the organic and inorganic analytes studied. Detection limits of 0.5 to 1.0 ppm were observed.