Excision of X-Ray-Induced Thymine Damage in Chromatin from Heated Cells

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Abstract
Experiments were performed to distinguish between 2 possible modes of hyperthermia-induced inhibition of thymine base damage excision from the DNA of CHO [Chinese hamster ovary] cells: heat denaturation of excision enzyme(s) or heat-induced alteration of the substrate for damage excision (chromatin). While hyperthermia (45.degree. C, 15 min) had no apparent effect on the capacity of the excision enzymes to excise damage from DNA, it had a dramatic effect (.apprx. 80% inhibition) on the ability of chromatin to serve as a substrate for unheated enzymes. Hyperthermia-induced radiosensitization of CHO cells may be due primarily to lesions in the cellular chromatin.

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