A SIMPLE METHOD FOR PRODUCING DIFFERENT GROWTH FRACTIONS IN VITRO FOR USE IN ANTI-CANCER DRUG STUDIES
- 1 January 1979
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 12 (1) , 11-16
- https://doi.org/10.1111/j.1365-2184.1979.tb00109.x
Abstract
A simple technique was used experimentally to produce in vitro Chinese hamster ovary [CHO] cells with growth fractions ranging from 0-100%. Known numbers of exponentially growing and plateau-phase tissue culture cells were mixed in various proportions to yield the desired final growth fraction. Cells attach to the culture flask surface within 1 h of mixing. Treatment at that time with the nitrosourea compounds, CCNU [1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea] and MeCCNU [1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea] [antineoplastic drugs], resulted in differential drug survival sensitivies that were dependent upon the growth fraction of the population treated.This publication has 7 references indexed in Scilit:
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