Effect of Threonylcarbamoyl Modification (t6A) in Yeast tRNAArgIII on Codon‐Anticodon and Anticodon‐Anticodon Interactions

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Abstract
The effect of N-[9-(β-d-ribofuranosyl) purin-6-ylcarbamoyl]threonine (t6A) adjacent to anticodon U*-C-U of yeast tRNAArgIII (where U* is a modified U), compared to its unmodified adenosine counterpart, has been evaluated by three independent methods: (a) the polynucleotide-directed binding of tRNA on ribosomes, (b) the ribosome-free trinucleotide binding to the anticodon. (c) the anticodon-anticodon binding test. The results obtained by these three methods indicate a small but significant stabilization effect of t6A on the binding of yeast tRNAArgIII with (a) poly(A,G) in the presence of Escherichia coli ribosomes, (b) free A-G-A triplet, and (c) E.coli tRNAServ (anticodon G-G-A). We therefore conclude that the stabilization effect of t6A occurs on U · A and U · G base pairs adjacent to the 5′ side of the modified nucleoside, most probably by stacking.