Glucose Biosensor Based on Immobilization of Glucose Oxidase in an Anionic Ion Exchange Polymer Blend
- 1 October 1992
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 25 (10) , 1835-1842
- https://doi.org/10.1080/00032719208018256
Abstract
A fast and simple procedure to prepare a glucose oxidase electrode is presented herein. A blend of poly[(4-vinylpyridine)-co-styrene] and a polystyrene with quaternized amine groups, named PolyXIO, solubilized in methanol has been used for the immobilization of glucose oxidase at the surface of a platinum electrode. The response of the biosensor is saturated at 20 mM of glucose and a linearity of current versus glucose concentration is observed up to 4 mM. With 5U of GOD entrapped in the polymer membrane, the maximum current reached 200 μA/cm2. The major advantages of this polymer blend lie in its good ionic diffusion capability, and because it has pendant groups opposite in charge to the outer groups on glucose oxidase.Keywords
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