A New Model to Study Hematopoietic Transplantation Antigens

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Abstract
Summary: A method for the study of hematopoietic transplantation antigens is described. It seems to fulfill the requirements for an immunochemical bioassay, i.e., to be simple, sensitive and quantitative. The procedure is as follows: a) Recipient mice are primed with varying doses of homologous donor antigens, and donors with sheep RBC antigen. b) Ten days later the recipients are exposed to 800 r and injected intravenously with 24 × 106 spleen cells of the donor, followed by intraperitoneal injection of sheep RBC. c) Anti-sheep RBC titer is determined 6 days later and compared with the titer of nonprimed controls. The RD50 (the dose of priming donor antigen capable of stimulating the host to reject 50% of the donor spleen cells) is 6 × 102 for viable bone marrow cells, 4 × 104 for bone marrow cells killed with x-rays, 1 × 105 for liver cells, and 5 × 105 for testicular cells. No priming potency was found with even 107 RBC.