Pyrazinamide Resistance and pncA Gene Mutations in Mycobacterium tuberculosis
Open Access
- 1 May 2008
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 52 (5) , 1852-1854
- https://doi.org/10.1128/aac.00110-08
Abstract
Thirty-four pyrazinamide-resistant and 37 pyrazinamide-susceptible Mycobacterium tuberculosis complex strains were analyzed for pncA gene mutations. None of the sensitive strains had any mutations, apart from silent mutations, whereas all but one resistant strain showed pncA mutations. By using sequencing as a means of early resistance detection, the inconsistency of phenotypic pyrazinamide assays can be circumvented.Keywords
This publication has 30 references indexed in Scilit:
- Nitrate reductase assay for the rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamideJournal of Antimicrobial Chemotherapy, 2007
- Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamideJournal of Antimicrobial Chemotherapy, 2007
- pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates from the southeast region of BrazilJournal of Antimicrobial Chemotherapy, 2006
- Evaluation of MGIT 960-Based Antimicrobial Testing and Determination of Critical Concentrations of First- and Second-Line Antimicrobial Drugs with Drug-Resistant Clinical Strains of Mycobacterium tuberculosisJournal of Clinical Microbiology, 2006
- Analysis of rpoB and pncA mutations in the published literature: an insight into the role of oxidative stress in Mycobacterium tuberculosis evolution?Journal of Antimicrobial Chemotherapy, 2005
- Characterization of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis in BrazilAntimicrobial Agents and Chemotherapy, 2005
- Evaluation of the Fully Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Pyrazinamide, Streptomycin, Isoniazid, Rifampin, and Ethambutol and Comparison with the Radiometric BACTEC 460TB MethodJournal of Clinical Microbiology, 2004
- Multicenter Evaluation of the MB/BACT System for Susceptibility Testing of Mycobacterium tuberculosisJournal of Clinical Microbiology, 2004
- Novel pncA mutations in pyrazinamide-resistant isolates of Mycobacterium tuberculosisMolecular Diagnosis, 1998
- Mutation in pncA is a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosisTubercle and Lung Disease, 1998