NEOPLASTIC PROGRESSION EVIDENCED IN THE L929 CELL SYSTEM .1. SELECTION OF TUMORIGENIC AND METASTASIZING CELL VARIANTS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 29  (3) , 269-279
Abstract
Starting with a L929 [mouse fibroblastic] cell strain of low tumorigenicity, and through in vivo and combined in vivo-in vitro manipulation, a number of related culture cell lines were isolated with different tumorigenicity and metastasis ability in C3HA/H mice. Within this system, tumorigenicity can be greatly enhanced without the simultaneous development of metastasis ability, the latter arising only after long-term in vivo serial transplantation. Metastatic variants express better the ability to undergo to cascade of metastasis than the primary tumor. The selection of metastatic variants from a tumor, using its s.c. inoculation, renders cell lines with different behavior. Finally, viable cells are recovered from lungs of animals inoculated with metastatic variants that would not be expected to show true metastatic foci; the results are commented on in relation to the phenomenon of metastasis dormancy. These experiments are discussed under the scope of the hypothesis of neoplastic progression as a multistep process.

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