THE EFFECT OF STRUCTURE ON NUCLEOSIDE KINASE ACTIVITY
- 1 October 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 45 (10) , 1619-1632
- https://doi.org/10.1139/o67-189
Abstract
A study was made of the in vitro phosphorylation of some nucleosides in cell-free extracts from a thioguanine-resistant subline of Ehrlich ascites carcinoma which lacked inosine monophosphate – guanosine monophosphate pyrophosphorylase. The results indicated that there were kinases which catalyzed the phosphorylation of a variety of nucleosides. Ribofuranosyl-6-thiopurme was phosphorylated whereas the xylofuranosyl-, arabinofuranosyl-, and lyxofuranosyl-6-thiopurine were not substrates. The xylose and ribose derivatives of 6-methylthiopurine were phosphorylated but the arabinose derivative was not. Guanosine and some of its analogs were also phosphorylated but no correlation could be found between structure and phosphorylation of the guanosine analogs tested.The reaction showed dependence on the addition of divalent cation and an ATP-regenerating system.This publication has 30 references indexed in Scilit:
- Structure-Activity Relationship of Some Purine 3'-Deoxyribonucleosides*Biochemistry, 1966
- Inhibition of formylglycinamide ribonucleotide synthesis in neoplastic cells by purines and analogsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Activity and Mechanism of Action of 6-Methylthiopurine Ribonucleoside in Cancer Cells Resistant to 6-MercaptopurineNature, 1965
- Formation of the mono-, DI- and triphosphate of cordycepin in ehrlich ascites-tumor cells in vitroBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1963
- Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk methodArchives of Biochemistry and Biophysics, 1961
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960
- Formation in vitro of deoxyadenosine triphosphate from deoxyadenosine in Ehrlich ascites cellsBiochemical and Biophysical Research Communications, 1960
- THE FORMATION OF 6-MERCAPTOPURINE RIBOSIDE PHOSPHATE IN ASCITES TUMOR CELLSCanadian Journal of Biochemistry and Physiology, 1959
- Thiation of Nucleosides. I. Synthesis of 2-Amino-6-mercapto-9-β-D-ribofuranosylpurine (“Thioguanosine”) and Related Purine Nucleosides1Journal of the American Chemical Society, 1958
- A comparison of the metabolism of purines and purine analogs by susceptible and drug-resistant bacterial and neoplastic cellsBiochimica et Biophysica Acta, 1957