Molecular Cloning and Genomic Analysis of Mouse GalNAc 2,6-Sialyltransferase (ST6GalNAc I)

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Abstract
CDNA clones encoding mouse GalNAc α2,6-sialyltransferase (ST6GalNAc I) were isolated from a mouse submaxdllary gland cDNA library. The deduced amino acid sequence of cDNA clones is 526 amino acids in length and has highly conserved motifs among sialyl transferases, sialyl motifs L, S, and VS. The expressed recombinant enzyme exhibited similar substrate specificity to chicken STGGalNAc L The mouse ST6GalNAc I gene was expressed in submaxillary gland, mammary gland, colon, and spleen. The mouse ST6GalNAc I gene was also cloned from a mouse genomic library, which was divided into 9 exons spanning over 8 kHobases of genomic DNA. The genomic structure of the mouse STGGalNAc I gene was similar to that of the mouse STGGalNAc II gene. Unlike the ST6GalNAc II gene, however, which has a housekeeping gene-like promoter with GC-rich sequences, the STBGalNAc I gene has two promoters and they do not contain GC-rich sequences but contain putative binding sites for tumor-associated transcription factors such as c-Myb, c-Myc/Max, and c-Ets. Analysis of the 5′-RACE PCR products suggested that the mouse STSGalNAc I gene expression is regulated by these two promoters in tissue-specific manners

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