Subunit Composition of Molecular Species of Legumin from the Broad Bean

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Abstract
The molecular species of legumin from broad bean seeds exhibit wide heterogeneity [S. Utsumi and T. Mori, Biochim. Biophys. Acta, 621, 179 (1980)]. The subunit compositions of these molecular species were analyzed according to the following procedure: the molecular species were separated by polyacrylamide gel electrophoresis, and then their subunit compositions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results indicated that five groups with nine kinds of submolecular species having different molecular weights and subunit compositions were present as legumin in the broad bean. According to these results, we have demonstrated the presence of another group consisting of two kinds of submolecular species, in addition to the four groups and seven kinds of submolecular species reported previously. Various possible molecular species of legumin composed of subunit groups classified according to size were presented.

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