The Metabolism of Cyclohexanol by Acinetobacter NCIB 9871

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Abstract
Acinetobacter NCIB 9871 was isolated by elective culture on cyclohexanol and grows with this compound as sole source of carbon. It displays a restricted growth spectrum, being unable to grow on a wide range of alternative alicyclic alcohols and ketones. Cyclohexanol‐grown cells oxidize the growth substrate at a rate of 230 μl of O2/h per mg dry wt with the consumption of 5.65 μmot of O2/μmol substrate. Cyclohexanone is oxidized at a similar rate with the consumption of 4.85 μmol of O2/μmol. 1 ‐Oxa‐2‐oxocycloheptane and 6‐hydroxyhexanoate are both oxidized at the same slow rate of 44 μl of O2/h per mg dry wt and adipate is not oxidized.Studies with cell extracts reveal the presence of inducible dehydrogenases for cyclohexanol, 6‐hydroxyhexanoate and 6‐oxohexanoate and a monooxygenase, that in conjunction with a lactonase converts cyclohexanone to 6‐hydroxyhexanoate. The monooxygenase is therefore presumed to be of the lactone‐forming type and the pathway for conversion of cyclohexanol to adipate; cyclohexanol → cyclohexanone → 1‐oxa‐2‐oxocycloheptane → 6‐hydroxyhexanoate → 6‐oxohexanoate → adipate; for which key intermediates have been identified chromatographically, is identical with the route for the oxidation of cyclohexanol by Nocardia globerula CL1.