Preparation and Biological Properties of Monoclonal Antibodies Against Human Erythropoietin
- 1 October 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 12 (5) , 599-608
- https://doi.org/10.1089/hyb.1993.12.599
Abstract
Several monoclonal antibodies (Mabs) against human erythropoietin (H-EPO) were obtained by fusion of myeloma cells with splenocytes from mice immunized with purified recombinant H-EPO. Three Mabs (D7, E14 and E73) were selected by radioimmunoprecipitation on the basis of their high affinity against H-EPO. Their dissociation constants were 0.3 nM for E14 and D7, and 17 nM for E73. The immunochemical properties of these Mabs were analyzed in respect to their capacity to react, to purify, and to inhibit the biological activities of H-EPO.Keywords
This publication has 17 references indexed in Scilit:
- Production of monoclonal antibodies against human erythropoietin and their use in the purification of human urinary erythropoietinJournal of Immunological Methods, 1988
- Characterization of erythropoietin receptor of murine erythroid cellsEuropean Journal of Biochemistry, 1987
- Characterization of recombinant human erythropoietin produced in Chinese hamster ovary cellsBiochemistry, 1987
- A modified ELISA that selectively detects monoclonal antibodies recognizing native antigenJournal of Immunological Methods, 1986
- Structural characterization of human erythropoietin.Journal of Biological Chemistry, 1986
- Isolation and characterization of an anti-peptide monoclonal antibody to human erythropoietin.Journal of Biological Chemistry, 1985
- Cloning and expression of the human erythropoietin gene.Proceedings of the National Academy of Sciences, 1985
- Isolation and characterization of genomic and cDNA clones of human erythropoietinNature, 1985
- Characterization of a monoclonal antibody to human erythropoietin.Proceedings of the National Academy of Sciences, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979