Studies on the Terminal Stages of Immune Hemolysis
Open Access
- 1 July 1979
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 123 (1) , 71-76
- https://doi.org/10.4049/jimmunol.123.1.71
Abstract
The inhibitory effects of 0.1 M EDTA on the lysis of E* prepared by incubating EA with whole GPC was studied. At high end point lysis (>70%) 0.1 M EDTA failed to prevent hemoglobin release whereas at lower end point (<60%) 0.1 M EDTA was effective. In all cases hemoglobin release was inhibited by 25% BSA. When E* were prepared by incubating EAC1-8 with C9, similar results were obtained. In this system the difference in the ability of 0.1 M EDTA to inhibit hemoglobin release at high or low end point lysis could not be correlated with the number of lesions/cell but appeared to be related to the C9 to SAC1-8 ratio. With limiting SAC1-8 and excess C9, E* were produced from which hemoglobin release could not be prevented by 0.1 M EDTA whereas at lower C9 to SAC1-8 ratios hemoglobin release was prevented by 0.1 M EDTA. These differences most probably reflect functionally different sized transmembrane channels that were produced at different C9 to SAC1-8 ratios.Keywords
This publication has 0 references indexed in Scilit: