Altered Erythrocyte Membrane Protein Phosphorylation in an Unusual Case of Hereditary Spherocytosis

Abstract
Membrane protein phosphorylation was examined in several members from a family with an unusual form of hereditary spherocytosis. After incubation of membrane ghosts with (.gamma.-32P) ATP, the phosphorylation of spectrin component II was diminished in the absence and presence of c[cyclic]AMP. In the presence of this nucleotide, the phosphorylation of components IV5 and IV8 was also decreased. Along with a previously reported alteration of a membrane neutral phosphatase in this family, these abnormalities remove the present condition from the usual form of hereditary spherocytosis.