Transport of mannose by an inducible phosphoenolpyruvate: fructose phosphotransferase system in Streptococcus salivarius

1 September 1994

journal article
Published by Microbiology Society in Microbiology

Vol. 140 (9) , 2433-2438
https://doi.org/10.1099/13500872-140-9-2433

Abstract

Streptococcus salivarius transports mannose by a phosphoenolpyruvate: sugar phosphotransferase system (PTS) which consists of a membrane Enzyme II and two forms of Enzyme III (III^Man) with molecular masses of 38.9 kDa (III^Man_H) and 35.2 kDa (III^Man_L) respectively. Using a pseudorevertant (strain 57P) isolated from a III^Man_L-deficient spontaneous mutant unable to grow on mannose, we demonstrated that S. salivarius could also transport mannose by an inducible fructose PTS. This PTS phosphorylated fructose at the C-1 position with a high affinity (10 μM) and mannose at the C-6 position with a low affinity (200 μM). Derepression of this system in some III^Man_L-deficient mutants would explain their ability to grow on mannose.

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