Delivery of foreign genes to intact barley cells by high-velocity microprojectiles
- 1 July 1989
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 78 (1) , 31-34
- https://doi.org/10.1007/bf00299749
Abstract
Foreign DNA was introduced through the cell walls of intact suspension culture cells of barley (Hordeum vulgare L.) by utilizing the particle acceleration approach. DNA-coated microscopic tungsten particles were accelerated to velocities that permitted their penetration of intact cells. Chimaeric constructs of β-glucuronidase and neomycin phosphotransferase II under the control of the dual Agrobacterium TR 1′2′ promoter or the cauliflower mosaic virus 35S promoter served as reporter genes. Three days after particle delivery, high-level expression of both reporter genes was observed. That plasmid size could be critical for stabilizing DNA in the course of particle delivery will be discussed.Keywords
This publication has 24 references indexed in Scilit:
- Chloroplast Transformation in Chlamydomonas with High Velocity MicroprojectilesScience, 1988
- Mitochondrial Transformation in Yeast by Bombardment with MicroprojectilesScience, 1988
- Genetically Transformed Maize Plants from ProtoplastsScience, 1988
- High-velocity microprojectiles for delivering nucleic acids into living cellsNature, 1987
- Transgenic rye plants obtained by injecting DNA into young floral tillersNature, 1987
- Agrobacterium-mediated delivery of infectious maize streak virus into maize plantsNature, 1987
- Stable transformation of maize after gene transfer by electroporationNature, 1986
- Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylaseNature, 1985
- A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extractsGene, 1984
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962