Isolation of a protein with cytokinin‐receptor properties by means of anti‐idiotype antibodies
- 26 February 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 261 (2) , 410-412
- https://doi.org/10.1016/0014-5793(90)80604-h
Abstract
A protein from cytosol of 10‐day‐old barley leaves with cytokinin‐receptor properties was isolated and 12 000‐fold purified by anti‐idiotype antibodies from anti‐BA serum. Cytokinin‐binding properties of this protein were demonstrated by its competition with Abba for immobilized BA in competitive ELISA. In the presence of BA the protein activates in vitro rRNA synthesis in the transcription elongation system containing chromatinbound RNA‐polymerase 1 from barley leaves. The protein with similar properties was isolated from barley leaf cytosol with BA‐Sepharose. The control proteins isolated with adenine‐ and ethanolamine‐Sepharose did not possess cytokinin‐binding properties and had no effect on RNA synthesis in vitro.Keywords
This publication has 10 references indexed in Scilit:
- Receptor-like cytokinin-binding protein(s) from barley leavesJournal of Plant Growth Regulation, 1988
- Making Antibodies Without the AntigensScience, 1985
- Resolution of the subunit composition of a cytokinin-binding protein from wheat embryosBiologia plantarum, 1985
- [40] Isolation of cytokinins by immunoaffinity chromatography and analysis by high-performance liquid chromatography radioimmunoassayPublished by Elsevier ,1985
- Phytohormones in the formation of crown gall tumorsPlanta, 1981
- Auxin ReceptorsAnnual Review of Plant Physiology, 1981
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnalytical Biochemistry, 1980
- [28] Enzyme immunoassay ELISA and EMITPublished by Elsevier ,1980
- Hormone Binding in PlantsAnnual Review of Plant Physiology, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970