Synthesis of [ 14 C]Cellobiose with Clostridium thermocellum Cellobiose Phosphorylase
- 1 October 1986
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 52 (4) , 902-904
- https://doi.org/10.1128/aem.52.4.902-904.1986
Abstract
Cellobiose labeled either at the reducing end (4- O -β- d -glucopyranosyl- d -[U- 14 C]glucopyranose) or at the nonreducing end (4- O -β- d -[U- 14 C]glucopyranosyl- d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to authentic cellobiose in melting point, optical rotation, isotopic dilution, and chromatographic properties.Keywords
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