A Desalting Approach for MALDI-MS Using On-Probe Hydrophobic Self-Assembled Monolayers
- 1 November 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (22) , 4716-4720
- https://doi.org/10.1021/ac970650u
Abstract
One of the problems encountered in preparing samples for matrix-assisted laser desorption/ionization (MALDI) analysis is the presence of nonvolatile salts in the sample. This difficulty is often exacerbated by the necessity to prepare the sample in the appropriate sample-to-matrix ratio. This paper reports a probe surface derivatization method that greatly simplifies this sample preparation process. By constructing self-assembled monolayers of octadecyl mercaptan (C18) on the MALDI probe surface, we were able to generate a surface capable of reversibly binding polypeptides via hydrophobic interactions, which in turn, permits the analyte to be easily concentrated and desalted directly on the probe tip.Keywords
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