LUNG GRANULOMATOUS HYPERSENSITIVITY TO EGGS OF SCHISTOSOMA JAPONICUM IN MICE ANALYSED BY A RADIOISOTOPIC ASSAY AND EFFECTS OF HYBRIDOMA (IDIOTYPE) SENSITIZATION

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Abstract
Summary: The radioisotopic assay for acute granulomatous hypersensitivity (AGH) to eggs of Schistosoma japonicum in mice sensitized with eggs in adjuvant has been examined in the high responder strain. C57BL/6. Responsiveness is expressed as the lung‐kidney ratio of radioactivity in mice challenged intravenously with eggs and injected with 123I‐iododeoxyuridine, Eggs vary in their AGH sensitizing and eliciting potency; eggs proven to be superior in the circumoval precipitation (COP) test for detection of human serum anti‐S. japonicum antibodies are superior in the C57BL/6 AGH assay. CBA/H are non responders and BALB/c are low to intermediate responders and are thus different from C57BL/6 mice. Short‐term infected CBA/H mice are low COP antibody responders relative to infected C57BL/6 and titres of IgM anti‐egg antibodies are low in the CRA/H strain as determined in a solid‐phase radioimmunoassay (RIA). Following egg sensitization. CBA/H mice are also lower responders than C57BL/6 mice in terms of antibodies with the specificity of a COP‐positive IgM hybridoma‐derived antibody, P.41, measured in a competitive RIA. No evidence has been obtained that alteration of the response to the target epitope of P.41 alters the responsiveness of C57BL/6 mice to S. japonicum eggs. Thus, large amounts of injected P.41 antibody do not alter lung AGH and induced anti‐idiotype responses to the P.41 protein do not influence AGH in egg‐sensitized C57BL/6 mice. However, immunization of C57BL/6 mice with another IgM anti‐schistosome hybridoma antibody, 1.39, results in partial inhibition of lung AGH responsiveness. The nature of the effect of 1.39 immunization on AGH to eggs remains unknown, but further analysis of the phenomenon may lead to novel approaches to the control of granulomatous inflammatory disease in high responders.

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