Mouse Antibody

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Abstract
Summary: It has been shown that peritoneal fluid of mice immunized intraperitoneally with Ea in Freund's adjuvants may contain as much as 2 mg of Ab N/ml. Qualitatively, mouse peritoneal fluid appears to be very similar to mouse serum. Mouse antibody behaves like rabbit antibody in the quantitative precipitin test; insoluble precipitates are formed in extreme antibody excess, and soluble complexes are formed in extreme antigen excess. The anti-Ea antibody, the only one studied, was found only in the γ-globulin portion of the fluid. Various factors affecting the precipitin reaction such as incubation temperature, length of incubation and NaCl concentration have been investigated, and conditions which permit satisfactory and reproducible results have been established.

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