Detection, distribution and probable fate of Escherichia coli O157 from asymptomatic cattle on a dairy farm
Open Access
- 1 August 1997
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 83 (3) , 297-306
- https://doi.org/10.1046/j.1365-2672.1997.00230.x
Abstract
The use of commercial anti‐Escherichia coli O157‐labelled magnetic beads was investigated to improve detection of E. coli O157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non‐sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non‐specific binding of non‐target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli O157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.Keywords
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