Multifiber, multiwavelength, fiber optic fluorescence spectrophotometer
- 1 January 1992
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Biomedical Engineering
- Vol. 39 (9) , 886-892
- https://doi.org/10.1109/10.256421
Abstract
A fiberoptic spectrofluorimeter was constructed capable of remote operation of up to 18 biosensors under fully automated, multiplexed control. Excitation and emission wavelengths were separately adjustable, as were optical bandwidths, signal gain, phase, integration time, and illumination duty cycle. Synchronous demodulation was used to enhance signal-to-noise ratio and allow operation under ambient lighting conditions. Detection limit for aqueous sodium-fluorescein was below 1 ng/mL at greater than unity signal-to-noise ratio, with effective sampling volumes less than 0.02 microL. The fluorimeter showed characteristics fiberoptic sampling behavior with asymptotic nonzero signal magnitudes under increasing pathlength conditions.Keywords
This publication has 11 references indexed in Scilit:
- Fiber Optic Sensor For Static Pressure MeasurementsPublished by SPIE-Intl Soc Optical Eng ,1988
- A fiber-optic interferometric seismometerJournal of Lightwave Technology, 1987
- A laser-pumped temperature sensor using the fluorescent decay time of alexandriteJournal of Lightwave Technology, 1987
- Fibre-optic sensors in biomedical sciencesPublished by Walter de Gruyter GmbH ,1987
- Single-mode fibre-optic magnetometer with DC bias field stabilizationJournal of Lightwave Technology, 1985
- Fiber Optic Sensors: An OverviewOptical Engineering, 1985
- An overview of fiber-optic gyroscopesJournal of Lightwave Technology, 1984
- Chemical sensors based on fiber opticsAnalytical Chemistry, 1984
- Optical fiber sensor technologyIEEE Journal of Quantum Electronics, 1982
- Fiber optic pH probe for physiological useAnalytical Chemistry, 1980