C‐terminal labelling of β‐casein
- 9 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (1) , 163-167
- https://doi.org/10.1016/0014-5793(87)81578-8
Abstract
This paper is the first to report specific labelling of a native protein at its C-terminal end by carboxypeptidase Y-catalyzed transpeptidation between β-casein and tritiated Phe amide. A tryptic digest of the radiolabelled protein was resolved by reversed-phase HPLC and a single labelled peptide was isolated therefrom. Sequence determination and FAB mass spectrometry showed that the last 2 residues (Val-209, Ile-208) of β-casein had been deleted and Ile 207 substituted by Phe, deamidation presumably occurring after transpeptidation. Identical results were obtained by transpeptidating the isolated C-terminal tryptic heptapeptide (203–209) of native β-caseinKeywords
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