RABBIT POLYMORPHONUCLEAR LEUKOCYTE MIGRATION IN VITRO IN RESPONSE TO LIPOPOLYSACCHARIDES FROM BACTEROIDES, FUSOBACTERIUM AND VEILLONELLA

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Abstract
Purified lipopolysaccharides (LPS) from strains of Bacteroides, Fusobacterium and Veillonella incubated with guinea pig serum, were tested for chemotactic activity against rabbit polymorphonuclear leukocytes (PMN) in modified Boyden chambers. Comparisons were made to a Salmonella LPS (S. enteriditis S-795). Submicrogram amounts of LPS induced positive chemotaxis, and a typical dose-response relationship up to certain dose levels was observed. The difference in chemotactic activity between the Veillonella LPS and LPS-S-795 was not statistically significant. The Fusobacterium LPS showed a non-significant or a highly significantly lower chemotactic capacity than LPS-S-795. The Bacteroides LPS were also clearly chemotactic, but considerably less when compared to the Salmonella LPS. When serum was not added, the LPS preparations showed no chemotactic activity.