Synthesis of 9-(?-d-arabinofuranosyl)guanine using whole cells of Escherichia coli
- 1 March 1990
- journal article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 32 (6) , 658-661
- https://doi.org/10.1007/bf00164735
Abstract
Synthesis of 9-(β-d-arabinofuranosyl)guanine (ara-G) from 1-(β-d-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2′-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%–65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%–53%.Keywords
This publication has 5 references indexed in Scilit:
- Chemical Conversion of Some Ribonucleosides into the Corresponding β-D-Arabinofuranosyl Derivatives1Nucleosides, Nucleotides and Nucleic Acids, 1983
- Preparation of 9-β-D-Arabinofuranosylguanine (araG)Nucleosides, Nucleotides and Nucleic Acids, 1982
- An enzymic synthesis of purine d-ArabinonucleosidesCarbohydrate Research, 1981
- A novel and simple method for the preparation of adenine arabinoside by bacterial transglycosylation reactionFEBS Letters, 1980
- Convenient Preparations of 9-β-D-Arabinofuranosylguanine, 9-β-D-Arabinofuranosylhypoxanthine and DerivativesSynthesis, 1978