A Quantitative Method for Determining Human γG Allotype Antigens (Gm)

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Abstract
A new quantitative method for determining relative concentrations of human Gm(a) and (f) allotype antigens is described. The technique employs automated equipment to measure the degree of inhibition of agglutination of an anti-Gm antiserum. Different concentrations of Gm(+) γG are assayed and a regression line is determined and graphed for each sample. Regression lines may then be compared quantitatively and characterized by their slopes. Quantitative comparison was made of the amount of protein required to produce 50% inhibition of hemagglutination in γG samples of different Caucasian phenotypes, each sample being taken from a pool of over 25 normal sera. In both Gm(a) and (f) systems, γG from homozygous subjects had approximately twice the antigenic strength as did γG from heterozygous persons. Subjects of different phenotypes produced Gm regression lines with similar slopes that are believed to reflect antigenic identity. In contrast, γG from a rhesus monkey exhibited a lower slope for Gm(f) indicating that the monkey possessed a cross-reacting antigen. Although high concentrations of Gm(-) γG had a weak ability to inhibit the Gm assay, the slope of the regression line was so low as to suggest that the reaction was nonspecific.

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